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“At 13 o'clock on December 16, 2016, in the experimental base of Zhongke Zhengbiao, Inner Mongolia Mizhen Cashmere Company, Industrial Park of Hangjinhouqi, Bayannaoer City, the ultra-fine cloned white cashmere goat was born here, and the little lamb was named as a mecca fan. This is the first case of somatic cell clones for the purpose of producing superfine white cashmere goats, which were purely experimental or purely transgenic in the past, marking an important step in the preservation and breeding of superfine cashmere goats in China. â€
On February 10, 2017, the technicians from the improvement station of the Municipality made a special trip to Zhongke Zhengbiao Experimental Base to inspect the growth of cloned cashmere goats. Rice fans, male lambs, ear mark A006, cashmere fineness of 13.89μm, birth weight 4.3kg, weight 8.5kg, good health. Its fellow brothers will be born in early April.
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Mica fan production process introduction
(I) Preliminary preparation
In order to restore the cashmere fineness quickly, Inner Mongolia Zhongke Zhengbiao Co., Ltd. and the Kunming Institute of Zoology, Chinese Academy of Sciences, have cooperated with each other. Since 2013, a general survey of cashmere goats has been carried out in the main production areas of Inner Mongolian cashmere goats, and blood and cashmere samples have been tested. The 14.5-micron fine cashmere goat basic ewes core group was used to complete transformation and production of ultra-fine cashmere goats with improved and rapid propagation techniques using genome-wide breeding, rapid embryo transfer and advanced technology integration.
(B) Establishment of cell lines
In 2015, Zhongke Zhengbiao purchased 6 rams of Inner Mongolia white cashmere goats from Alashan League Alashanyouqi, and finally determined a fineness of 13.8um, cashmere length of 6.5cm, cashmere yield of 1100g through the determination of production performance. The 3-year-old adult ram was a donor sheep and ear cells were collected at that time. However, it was affected by the seasonal estrus of cashmere goat ewes and seasonal killing of cashmere goats at local slaughterhouses. It was not collected until early 2016. The high-quality Erlangshan white cashmere goat oocytes completed the establishment of a cloned sheep cell line in February 2016.
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(c) Embryo transfer
In mid-July 2016, technicians implanted 15 A- and B-grade embryos that were cultured in vitro to the blastula stage and were implanted into the oviducts of 2 recipient ewes by surgery. Each of them was placed on 7 and 8 of them respectively. Embryo.
(D) The birth of the first ultrafine clone goat
On December 16, 2016, a recipient ewe gave birth. Due to the large size of the lamb, the technicians took a caesarean section for the ewe to prevent childbirth. Finally, the lamb was born successfully. The lamb was named after the rice coffee fan.
(E) The second batch of ultra-fine clone cashmere goat embryo transfer
At the beginning of November 2016, the second batch of Miga fans and cell cloned embryos were successfully transplanted into the uterus of 50 recipient ewes. The ewes are in good conditions of pregnancy and are expected to lamb at the beginning of April.
(VI) Factory production of clones
In 2017, Zhongke Zhengbiao planned to transplant the third batch of Miga fans with their cell cloned embryos into the uterus of 1500 recipient ewes. At that time, 500 clone goats will be born.
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According to reports, Zhongke Zhengbiao has reached a strategic agreement with Baotou Luwang Group to build two breeding bases, a cashmere goat research institute and a cashmere acquisition center in Bayannur City in the next three years. Among them, the Sun Temple Breeding Base in Hangjinhouqi will be mainly self-cultivation and self-cultivation, and it will be mainly used for breeding cloned cashmere goats. The project plans to use 1,000 mu of land and has allocated 200 mu. It is planned to start a civil engineering project in the first year of this year; the Tonghetai breeding base in Urad Zhongqi will be carried out in the mode of government + enterprise + farmer households. The government allocates 10,000 mu of grassland and 6 breeding stocks that have been built. Field, the company's investment management, the realization of state-owned assets to maintain value-added efficiency, has now reached a preliminary agreement with the Urad Zhongqi flag government; Cashmere Goat Research Institute has been registered in 2016, plans to start a civil engineering project during the year; Urad Middle The flag cashmere acquisition center mainly adopts the pattern of sheep cash collection to reduce the cost of enterprise cashmere goat breeding and guarantee the initiative of high quality cashmere acquisition. The cashmere goats raised on behalf of the farmers were all implanted with electronic ear tags to ensure the traceability of the cashmere products.
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